Invitrogen maxiprep manual

be performed by Invitrogen (part of Life Technologies). In an emergency, immediately turn the power switch Off and unplug the adapter and instrument. Additional Safety The BenchPro® instrument eliminates routine manual processing steps for maxiprep plasmid DNA purification while maintaining a similar performance. Plasmid DNA Miniprep, Midiprep, and Maxiprep Kits. The PureLink ™ Nucleic Acid Purification Rack consists of a Column Holder Rack (for processing 12 miniprep, 8 midiprep, and 4 maxiprep columns), a Collection Tube Rack (capable of accommodating various types and sizes of recovery tubes), and a large capacity Waste Tray for collecting waste. Description. The PureLink HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Maxiprep Kit protocol will typically yield –μg plasmid DNA from –mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients.

User Manual Corporate Headquarters Invitrogen Corporation Faraday Avenue Carlsbad, CA T: 1 7F: 1 7E: www.doorway.rue@www.doorway.ru For country-specific contact information visit our web site at www.doorway.ru Bac-to-Bac® TOPO® Expression System An efficient cloning and site-specific transposition system to. The following diagram is a simplified depiction of the maxiprep plasmid isolation procedure using a vacuum manifold. For detailed instructions on performing the protocol, see “Maxiprep procedure (vacuum manifold)“ on page Vacuum protocol workflow Product information Workflow 10 PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification. Plasmid DNA Miniprep, Midiprep, and Maxiprep Kits. The PureLink ™ Nucleic Acid Purification Rack consists of a Column Holder Rack (for processing 12 miniprep, 8 midiprep, and 4 maxiprep columns), a Collection Tube Rack (capable of accommodating various types and sizes of recovery tubes), and a large capacity Waste Tray for collecting waste.

2) Alternatively, use the QIAGEN maxiPrep Kit protocol using (2X) the volume of P1, P2 and P3 Buffers. 3) Digest μg of minicircle plasmid with. XLGold Transformation Protocol. Growth medium for AD cells§ [Invitrogen Life Technologies (Gibco) Catalog #]. 3 авг. г. This new plasmid DNA isolation protocol will significantly reduce time Lipofectamine (Invitrogen, Grand Island, NY, USA) and 2 ug.